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CELLULAR BIOTECHNOLOGY
ADRIANA CANAPA

Seat Scienze
A.A. 2016/2017
Credits 6
Hours 48
Period 1^ semestre
Language ENG
U-gov code SM04 5S004

Prerequisites

Knowledge of the basic concepts of cytology and histology, genetics and molecular biology.

Development of the course

The lectures are planned (5 CFU) as well as the preparation and presentation of scientific papers related to the topics covered during the lectures and also the laboratory practices (1 CFU).

Learning outcomes

Knowledge:
At the end of the course the student will have acquired the knowledge on basic procedures useful for in vitro growing of eukaryotic cells and on the methodologies to carry out their genetic manipulation for medical and industrial applications.

Ability to apply the knowledge:
The student will be able to design strategies for biotechnological compounds for pharmaceuticals and food, for monoclonal antibody production, and for obtaining genetically modified organisms from isolated cells or cell cultures.

Soft skills:
Through the laboratory practice and the preparation and presentation of reports on scientific articles students will acquire autonomy in judgment as well as communication skills also arising from work in groups.

Program

Objectives and instruments for cell biotechnologies. Cell and tissue cultures. Stem cells. Monoclonal antibody production. Tissue engineering and clinical applications. Recombinant DNA technology. Production of recombinant proteins in eukaryotic cells. Site-directed mutagenesis. Techniques for the production of transgenic animals. Cloning of animals by transfer of the nucleus. Gene therapy applied to human. Cell biotechnology applications in the various fields.

Development of the examination

Methods for assessing learning outcomes:
Oral exam after registration in the reserved area.

Criteria for assessing learning outcomes: 
The oral examination is aimed at assessing the knowledge and understanding of cellular biotechnology and other subjects needed to resolve problems related to course objectives.

Criteria for measuring learning outcomes:
The final mark is assigned exclusively on the oral exam  through questions at low, medium, and high difficulty.

Criteria for conferring final mark:
The final mark is assigned on the basis of the student's ability to provide answers that demonstrate mastery of the subject with clarity and with a relevant technical and scientific terminology. It will also evaluate the student's ability to link the topics covered during the course and the presentation of the scientific paper.

Recommended reading

Slides provided during the course.

Courses

• Biologia molecolare e applicata